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hepg2 cell culture supernatants  (Novus Biologicals)


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    Novus Biologicals hepg2 cell culture supernatants
    Hepg2 Cell Culture Supernatants, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 93/100, based on 11 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/hepg2+cell+lysate/HepG2+Whole+Cell+Lysate/pmc11296239__BLOODA_ADV___2023___012228___mmc1-3-5-18
    Average 93 stars, based on 11 article reviews
    hepg2 cell culture supernatants - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Transfection:

    Article Title: Endogenous IGFBP-3 Mediates Intrinsic Apoptosis Through Modulation of Nur77 Phosphorylation and Nuclear Export.
    Article Snippet: Mirus Transit TKO transfection reagent was purchased from Stratagene.Mirus Transit TKO transfection reagent was purchased from Stratagene.. HepG2 cell lysate was obtained from Novus Biologicals.. Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.

    Isolation:

    Article Title: Endogenous IGFBP-3 Mediates Intrinsic Apoptosis Through Modulation of Nur77 Phosphorylation and Nuclear Export.
    Article Snippet: Mirus Transit TKO transfection reagent was purchased from Stratagene.Mirus Transit TKO transfection reagent was purchased from Stratagene.. HepG2 cell lysate was obtained from Novus Biologicals.. Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.

    Quantitative RT-PCR:

    Article Title: Endogenous IGFBP-3 Mediates Intrinsic Apoptosis Through Modulation of Nur77 Phosphorylation and Nuclear Export.
    Article Snippet: Mirus Transit TKO transfection reagent was purchased from Stratagene.Mirus Transit TKO transfection reagent was purchased from Stratagene.. HepG2 cell lysate was obtained from Novus Biologicals.. Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.

    Western Blot:

    Article Title: Endogenous IGFBP-3 Mediates Intrinsic Apoptosis Through Modulation of Nur77 Phosphorylation and Nuclear Export.
    Article Snippet: Mirus Transit TKO transfection reagent was purchased from Stratagene.Mirus Transit TKO transfection reagent was purchased from Stratagene.. HepG2 cell lysate was obtained from Novus Biologicals.. Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.

    Control:

    Article Title: Endogenous IGFBP-3 Mediates Intrinsic Apoptosis Through Modulation of Nur77 Phosphorylation and Nuclear Export.
    Article Snippet: Mirus Transit TKO transfection reagent was purchased from Stratagene.Mirus Transit TKO transfection reagent was purchased from Stratagene.. HepG2 cell lysate was obtained from Novus Biologicals.. Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.

    Membrane:

    Article Title: Endogenous IGFBP-3 Mediates Intrinsic Apoptosis Through Modulation of Nur77 Phosphorylation and Nuclear Export.
    Article Snippet: Mirus Transit TKO transfection reagent was purchased from Stratagene.Mirus Transit TKO transfection reagent was purchased from Stratagene.. HepG2 cell lysate was obtained from Novus Biologicals.. Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.Cell culture The bovine MEC line MAC-T (23) was routinely maintained in complete media consisting of DMEM-H, 20 U/mL penicillin, 20 g/mL streptomycin, 50 g/mL gentamicin, 10% FBS, and 5 g/mL insulin.



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    Image Search Results


    Characterization of iHeps generated from PH1 patient-derived fibroblasts and AGXT gene corrected clones (A) Expression of hepatocyte specific genes in iHeps generated by direct cell reprogramming from PH1 patient-derived fibroblasts and AGXT gene corrected fibroblasts. The expression of genes involved in different liver functions was analyzed by RT-qPCR. Data from twenty-six independent experiments, conducted with two different PH1-donor fibroblasts (PH1-1 (n = 8) and PH1-2 (n = 4)), three AGXT point mutation corrected clones from the two PH1 donors (GC-1A (n = 4) and GC-1C (n = 1) derived from PH1-1. GC-2A (n = 2) derived from PH1-2) and four AGXT targeted knockin clones (pA-1A (n = 2), pB-1C (n = 2), and pD-1A (n = 2)), derived from PH1-1. pD-2A (n = 1) derived from PH1-2). Violin plot of data show median (bold dashed line) and interquartile range (light dashed line). Normal distribution of the data was assessed by a Kolmogorov-Smirnov test. Nonparametric Kruskal-Wallis with Dunn’s multiple comparisons test was used to compare, for each analyzed gene, iHeps with hepatocytes ($) or with fibroblasts (#). Significant differences between cell populations are marked as follows: $/#: p < 0.05; $$/##: p < 0.01; $$$/###: p < 0.001; $$$$/####: p < 0.0001. ABCC2: ATP binding cassette subfamily C member 2, CP: ceruloplasmin, FGL1: fibrinogen-like protein 1 and HP: haptoglobin. (B) Glycogen storage in the generated iHeps. Microscope images of PAS staining on starting human fibroblasts and generated iHeps, from healthy donor, PH1 patients and AGXT -corrected clones (GC-1A from PH1-1 and GC-2A from PH1-2). HepG2 cell line was used as positive control. All images were taken with 100× magnification. Scale bar: 75 μm.

    Journal: iScience

    Article Title: Restored glyoxylate metabolism after AGXT gene correction and direct reprogramming of primary hyperoxaluria type 1 fibroblasts

    doi: 10.1016/j.isci.2024.109530

    Figure Lengend Snippet: Characterization of iHeps generated from PH1 patient-derived fibroblasts and AGXT gene corrected clones (A) Expression of hepatocyte specific genes in iHeps generated by direct cell reprogramming from PH1 patient-derived fibroblasts and AGXT gene corrected fibroblasts. The expression of genes involved in different liver functions was analyzed by RT-qPCR. Data from twenty-six independent experiments, conducted with two different PH1-donor fibroblasts (PH1-1 (n = 8) and PH1-2 (n = 4)), three AGXT point mutation corrected clones from the two PH1 donors (GC-1A (n = 4) and GC-1C (n = 1) derived from PH1-1. GC-2A (n = 2) derived from PH1-2) and four AGXT targeted knockin clones (pA-1A (n = 2), pB-1C (n = 2), and pD-1A (n = 2)), derived from PH1-1. pD-2A (n = 1) derived from PH1-2). Violin plot of data show median (bold dashed line) and interquartile range (light dashed line). Normal distribution of the data was assessed by a Kolmogorov-Smirnov test. Nonparametric Kruskal-Wallis with Dunn’s multiple comparisons test was used to compare, for each analyzed gene, iHeps with hepatocytes ($) or with fibroblasts (#). Significant differences between cell populations are marked as follows: $/#: p < 0.05; $$/##: p < 0.01; $$$/###: p < 0.001; $$$$/####: p < 0.0001. ABCC2: ATP binding cassette subfamily C member 2, CP: ceruloplasmin, FGL1: fibrinogen-like protein 1 and HP: haptoglobin. (B) Glycogen storage in the generated iHeps. Microscope images of PAS staining on starting human fibroblasts and generated iHeps, from healthy donor, PH1 patients and AGXT -corrected clones (GC-1A from PH1-1 and GC-2A from PH1-2). HepG2 cell line was used as positive control. All images were taken with 100× magnification. Scale bar: 75 μm.

    Article Snippet: Three hundred micrograms of iHeps lysates and 5μg of HepG2 cell lysates were pre-incubated first with horseradish peroxidase (HRP)-conjugated donkey anti-rabbit IgG 1:5000 (Amersham, Cat#NA934), and second with the Protein A/G PLUS reagent (Santa Cruz Technologies, Cat#sc-2003).

    Techniques: Generated, Derivative Assay, Clone Assay, Expressing, Quantitative RT-PCR, Mutagenesis, Knock-In, Binding Assay, Microscopy, Staining, Positive Control

    Journal: iScience

    Article Title: Restored glyoxylate metabolism after AGXT gene correction and direct reprogramming of primary hyperoxaluria type 1 fibroblasts

    doi: 10.1016/j.isci.2024.109530

    Figure Lengend Snippet:

    Article Snippet: Three hundred micrograms of iHeps lysates and 5μg of HepG2 cell lysates were pre-incubated first with horseradish peroxidase (HRP)-conjugated donkey anti-rabbit IgG 1:5000 (Amersham, Cat#NA934), and second with the Protein A/G PLUS reagent (Santa Cruz Technologies, Cat#sc-2003).

    Techniques: Recombinant, Virus, Derivative Assay, Cell Culture, PCR Cloning, Reverse Transcription, SYBR Green Assay, Lactate Dehydrogenase Assay, Sequencing, Knock-In, Plasmid Preparation, Amplification, Gene Expression, Software, Extraction